Polom, M. full-sized slides of breast malignancy resections biopsies obtained for diagnostic purposes. For digital analysis slides were pre-screened at 20 and 100 magnification for all Caspofungin Acetate those fluorescent signals and semi-automated scoring was performed on at least two pictures with the D-Sight HER2 FISH analysis module. Results were compared to data obtained previously with the manual Abbott FISH test. Results: The overall agreement with Abbott FISH data among TMA samples and in 50 selected IHC++ cases was 98.8?% (kappa = 0.94) and 93.8?% (kappa = 0.88), respectively. The results of 50 unselected IHC cases were concordant with previously obtained IHC and/or FISH data. Conclusion: The combination of the Leica FISH system with the Menarini Benelux D-Sight digital imaging platform is feasible for the assessment of HER2 status in routine clinical practice in patients with invasive breast cancer. OFP-01-002 A new quantitative in-situ immunohistochemistry method H. Derand *, J. Lohse, K. Petersen, K. Jensen, R. J?rgensen *Dako A/S, Research and Development, Glostrup, Denmark Objective: There is a general need to make pathologic examinations less subjective and to support the accuracy required for companion diagnostics. The aim of this study was to develop a new IHC method based on bright field technology combining morphological information with quantitative assessment. Method: By chemical manipulation of a visualization system, single antibodies are Caspofungin Acetate visualized as dots instead of a conventional stain. The dots can easily be counted by image analysis, and the number of dot reflects protein expression levels. Initial assay performance was evaluated using Her2 as a test system; breast malignancy cell lines as well as breast cancer tissue specimen were included. Results: The assay generates highly reproducible results and gives a linear assay with a larger dynamic range than the conventional assays. No overlap was seen between the different breast malignancy cell lines. Moreover, evaluation of HER2 status in a number of Caspofungin Acetate breast cancers by the use of this new method showed a strong correlation with established methods. Conclusion: The data suggest enumeration of dots can be direct related to protein expression levels. This represents a new and standardized way of objectively determining protein amounts in cells and tissue in situ. OFP-01-003 Automated image analysis enables accurate enumeration NOX1 of the Ki-67 labelling index of breast malignancy A. Laurinavicius *, A. Laurinaviciene, R. Meskauskas, I. Baltrusaityte, J. Besusparis, P. Herlin, B. Plancolaine, N. Elie, P. Belhomme, C. Bor-Angelier *National Center of Pathology, Vilnius, Lithuania Objective: Immunohistochemical Ki67 evaluation reflects proliferative activity and is regarded as important prognostic/predictive marker of breast cancer. However, its potential is usually hindered by lack of standardized and efficient methodologies to measure the Ki67 expression. Besides many other aspects, key element of the methodology remains accurate enumeration of Ki67-labelling index (LI). We investigated the accuracy aspect of automated image analysis (IA) approach. Method: TMA (1?mm diameter spot per patient, value?=?0.007. Conclusion: The incidence of synchronous endometrial and ovarian cancer is not negligible, is commonly seen with malignant endometrioid ovarian tumors. Percentage of recurrence increased Caspofungin Acetate in the cases of ovarian tumor coexisting with SUC than in isolated ovarian tumor, ovarian tumors coexisting with (SUC) show a significant increase in Her2 over expression predicting an underlying molecular genetic defect causing this association. OFP-10-003 Ovarian metastasis of endocervical adenocarcinoma P. Caseiro Silverio *, H. Kannuna, D. Huber, J.-C. Tille *H?pital Universitaire Genve, Switzerland Objective: Ovarian metastasis of endocervical Caspofungin Acetate adenocarcinoma is uncommon and can mimic primary ovarian neoplasm. Results: A 51-year-old woman was hospitalized for thromboembolic disease progression despite anticoagulation. Computer tomography showed a suspicious pelvic tumor and an elevated CA 125. Exploratory laparoscopy confirmed a 12?cm left adnexal mass with moderate ascite and no other intra-abdominal lesions. Peritoneal biopsies were unfavorable and peritoneal cytology.