The cell pellets was suspended within a sodium phosphate buffer (50 mm, pH 7.0) with lysozyme before sonication. DNA of (ATCC amount: BA.A.-334D-5). The forwards primer was 5-TATATACATATGGAGTCTA.A.ACCAGCAGCAGA.A.GC-3, as well as the change primer was 5-GCGCGCCTCGAGTTCTTCTGTCATCTTTTGGA.A.CGG-3. NdeI and XhoI site (underlined) had been put into the forwards and invert primers, respectively. The cDNA fragment of …
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