Briefly, the recombinant antigen was coated to the plate in concentrations of 0.5C2 g/mL. a prokaryotic manifestation plasmid, which was induced by IPTG to express soluble pp62 protein. Western blot analysis showed that it experienced great reactivity. Using the purified recombinant protein as an antigen, an indirect ELISA method for detecting ASFV antibody was founded. …
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