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A.S.-C. represent the mean??SEM (n?=?5C6 individual experiments, 3C4 wells/experiment). Values that do not share a TR-14035 common letter (A,B and C) are statistically different. Interaction of leptin, adiponectin and resistin with primary regulators of somatotrope function in baboon cell cultures The mechanisms that regulate somatotrope function are complex, for multiple central and peripheral factors can directly and indirectly control and modulate, alone or in conjunction, GH expression and secretion8. Therefore, and TR-14035 based on the results showed in Fig. 1, we sought to determine the potential interaction between leptin, adiponectin, and resistin with the primary regulators of GH secretion (i.e. GHRH, ghrelin and SST49,50,51,52) after a 4h-incubation. As previously observed, leptin and resistin alone stimulated, while adiponectin decreased basal GH release (Fig. 2). Moreover, as shown earlier49,50,51, GHRH and ghrelin alone (10?nM) stimulated GH release in baboon cell cultures, whereas SST alone (100?nM) tended to decrease basal GH release (although this latter effect did not reach statistical significance) (Fig. 2). Notably, comparison of the stimulatory effect of leptin or resistin with GHRH or ghrelin revealed that the effects of these two adipokines were slightly, but significantly, less intense than that evoked by GHRH or ghrelin (176%, 157%, 206% and 220%, respectively; control set at a 100%; Fig. 2). Open in a separate window Figure 2 Effect of 4?h treatment of GHRH (10?nM), ghrelin (10?nM) and SST (10?nM) in absence or presence of leptin (10?ng/ml), adiponectin (10?nM) or resistin (0.1?nM) on GH secretion in primary pituitary cell cultures from baboons.Data are expressed as percent of control (set at 100%) and represent the mean??SEM (n?=?4 individual experiments, 3C4 wells/experiment). Values that do not share a common letter (A,B,C and D) are statistically different. Co-incubation of leptin or resistin with GHRH and ghrelin did not alter the stimulatory actions of GHRH/ghrelin on GH secretion from primary pituitary cell cultures of baboons (Fig. 2), suggesting that leptin and resistin could trigger common intracellular signaling pathways with GHRH and ghrelin to stimulate GH release (as discussed further below). Previous data available, derived from early studies conducted in non-primate species (i.e. ovine, bovine, pig and rat) have shown that leptin can either inhibit29,53, stimulate21, or have no effect26,29,54 on GHRH-stimulated GH release from cultured anterior pituitary cells. These discrepancies may be due, in part, to the time of incubation (short vs. long periods), cell preparation (i.e. primary cell cultures, explants, etc.), culture conditions, and/or age studied, but also, most likely, to fundamental differences in the physiology of somatotropes from different species. Nevertheless, to our knowledge, this is the first report on the direct interaction between leptin and ghrelin, or between resistin and GHRH or ghrelin, at the anterior pituitary level using primary pituitary cultures of a normal, intact, cellular model. However, it should be mentioned that further support for a direct interaction between leptin and ghrelin at the pituitary level was originally provided by data showing that ghrelin treatment alone, or in combination with GHRH, stimulated or rescued GH store and/or secretion in the pituitary of a mutant mouse model lacking leptin receptor from somatotropes to the normal levels found in the control-intact model, suggesting that pituitary ghrelin is involved in optimizing the somatotrope responsiveness to primary regulators of somatotrope function55. Remarkably, this is also the first report demonstrating that SST is capable to directly block the stimulatory actions of both adipokines, leptin and resistin, on GH release (Fig. 2), which might suggest the existence of a putative association between SST levels and the leptin- and resistin-induced GH release at the pituitary level. In direct support of this TR-14035 notion, a previous study showed that the direct stimulatory actions of leptin on GH secretion required a reduction in the SST tone from porcine cultured median eminence-pituitaries co-incubated with the anterior pituitary cells26. In contrast, adiponectin was able to fully block the stimulatory actions of GHRH, but not ghrelin, on baboon GH secretion. These observations are opposite to those previously published by our group using primary pituitary cell cultures of rats32,33, which showed that, although treatment with adiponectin alone stimulated GH release from rat pituitary cell cultures [similar observation to the present study with baboon cell cultures (Figs 1 and ?and2)],2)], when co-incubated, adiponectin blocked the stimulatory effect of ghrelin, but not GHRH, on rat GH secretion. Hence, the differences between these two studies, together with the discrepancies discussed previously on the co-administration of leptin and GHRH, would suggest that the interactions of leptin or adiponectin with LRCH1 the primary positive regulators of GH release (i.e. GHRH and ghrelin) are not fully conserved across species. Notwithstanding, in support of our observation of.